FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19914096
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG4932012.476642675620324TruSeq Adapter, Index 13 (97% over 39bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1062150.5333659132706803No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA959990.48206556802779293No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC816350.40993575605942645No Hit
CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT635900.31932154992122164No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT527590.26493293996373224No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC427320.21458167119411292No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT381520.19158288681544972No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC361850.18170546129736442No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC357230.1793854965849316No Hit
CGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAG325560.16348218869689088No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT324000.16269882398879668No Hit
AGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGA319680.16052950633561272No Hit
CCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACT309090.15521166514412707No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC298980.1501348592474396No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA281440.1413270278500214No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA273020.13709886705376936No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG271010.1360895317568018No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT266290.13371935135795268No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG259550.13033481409349437No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA244670.12286271995474965No Hit
TTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGG243580.12231536897281202No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG240250.12064318661514939No Hit
CGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC239420.12022639641789414No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA234850.11793153954866945No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT233030.11701761405589287No Hit
TCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAA226480.11372848659562554No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC226270.11362303365415131No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC224550.11275932384779103No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAAACTCGTATG223810.11238772776831045TruSeq Adapter, Index 13 (97% over 39bp)
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT220820.11088627874446322No Hit
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAA219350.11014810815414369No Hit
TCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTG211360.10613587480948168No Hit
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC207600.10424776500022898No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCA203260.10206840420976176No Hit
GTCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA201590.10122980224660963No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG58050.044.9562953
GTTACGA69800.042.8622971
AGCACAC1917050.042.3468749
TTACGAC70500.041.9843522
GAGCACA1998250.040.727658
ACGACTT132250.039.134781
TGTCGAG35000.038.9369135
TACGACT77850.037.991973
AGAGCAC2159050.037.757837
CGGAAGA2196450.037.1333
AAGAGCA2202350.037.0187726
TCGGAAG2255200.036.1831782
GAAGAGC2390750.034.1985865
GTCGGTA13500.033.970021
GTCGAGG42900.032.2736246
GGAAGAG2536200.032.2639244
ATCGGAA2615900.031.4167921
CTTTAAG325200.029.7036231
TCTTAAC511650.028.5421872
AACGGTT514750.028.319626
Produced by FastQC (version 0.11.5)