FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20566569
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1057550.5142082765482177No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA557140.27089593796612355No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT452440.21998807871162177No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC373640.18167347212848192No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC315840.15356961095455446No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG279850.13607033822705186No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC279730.1360119911104278No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC279020.13566677067040206No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA277020.13469431872666754No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT272540.1325160263727022No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC239170.11629066569149185No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC224350.1090847967884191No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA221980.1079324412350937No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG212560.10335219258010414No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT212370.10325980964544937No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA99350.046.111864
ACGACTT72150.044.605311
CCCGTAT108800.044.000661
TATCGAA109600.042.1278275
GTTACGA26150.040.193864
TTACGAC26350.039.061415
TACGACT27950.037.085486
CGTATCG167000.032.691743
TGTCGAG29350.031.1173535
ACCCGGG761950.029.077301145
CGACTTT136900.027.1312
AACGGTT328200.026.7899516
TGTTACG39800.026.5967923
GAACTCT866650.026.170479
CCTCTTG809700.025.4214743
GTCGAGG36300.025.3598566
TCTTAAC350400.025.3198812
TTGAACT927800.025.0159287
CCCGTCG73300.024.6906191
GTCGGTA16900.024.6134431
Produced by FastQC (version 0.11.5)