FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19601610
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1371630.6997537447179083No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA709140.3617764051014177No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC507110.25870834079445515No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT494320.252183366570399No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA451820.230501474113606No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC450900.23003212491218833No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC381380.19456565047462937TruSeq Adapter, Index 2 (100% over 50bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC316490.16146122690942225No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC308320.15729320193596344No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC302830.1544924115927212No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT302700.15442609050991218No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA294020.14999788282697185No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC272650.13909571713752084No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC265240.13531541541740702No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT264380.13487667594651664No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC263080.1342134651184265No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG246870.12594373625431787No Hit
CTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCA227130.11587313491085681No Hit
GTGATGTTATAAGCCTAAGGCAGCTTGACTTGCAGCAACAAGTTTTTTAC223570.11405695756624074No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC218610.11152655317598911No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA204540.10434857136735196No Hit
CTTCATTTTCTTTCTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATG204260.10420572595822486No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT203490.1038129010831253No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA201770.10293542214134452No Hit
GTCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA197400.10070601343461073No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTT77800.050.075381
GTATCGA70950.043.7267884
CCCGTAT78350.040.319161
GTTACGA26600.038.6957174
TGTTACG27250.037.78063
TATCGAA85350.036.1773535
CCCGTCG87150.034.071371
TTACGAC30800.033.887735
GTCGGTA19450.033.0084881
GAACTCT936600.032.3649529
CCTCTTG846250.032.15343
ACCCGGG919300.031.919847145
TGTCGAG24250.031.6828925
CTTGAAC954850.031.222576
TTGAACT996050.030.9717377
TCTTGAA939950.030.6754345
TCTTAAC336500.030.589992
CTCTTGA915100.030.4726224
CGCGTAT2651.2914825E-1030.2837311
AACGGTT341700.030.1223246
Produced by FastQC (version 0.11.5)