FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19527950
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA427910.21912694368840557No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT382370.19580652347020552No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC332410.17022268082415204No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT311950.15974539058119258No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT265770.1360972349888237No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG253410.12976784557518836No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA243320.12460089256680809No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT234860.120268640589514No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC223930.11467153490253713No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC222120.11374465829746595No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG220590.1129611659185936No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG215590.1104007333078997Illumina Single End PCR Primer 1 (100% over 50bp)
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA205690.10533107673872577No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA201450.10315982988485733No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG130550.049.415443
AGTTACG103350.046.847282145
GTCTAAC141850.046.0561451
CGACCGT33600.043.648991
ACGTGCG160250.040.263618
TAACACG161200.040.0200734
AACACGT161100.039.997875
ACCCGGC167150.038.940425
GAGTCTA138050.038.497232
AGTCTAA140700.037.7623863
ACACGTG178450.036.2257846
CGTGCGC190450.034.222069
TCAGACG203150.034.0124552
CAGACGT200550.033.9746323
CACCCGG200950.032.21194
AGACGTG213800.031.8015334
TCTAACA211000.030.9945962
ACCGTTG47600.030.6082633
CACGTGC222400.029.2045567
GACCGTT51400.028.4937862
Produced by FastQC (version 0.11.5)