FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19727418
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA786560.39871411453845607No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA352370.17861942196388803No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT262770.13320040159335603No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC221550.11230562458807332No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC210490.10669921426108576TruSeq Adapter, Index 7 (100% over 50bp)
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC199220.10098635310510479No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGG477950.031.8893145
ACGACTT53300.028.2712271
GTATCGA48150.027.8517424
CTTAACG208200.026.3919143
CGTATCG83050.026.011123
GAACTCT614100.026.0022229
TTCGCGC25400.025.9702287
AACGGTT213150.025.5741166
TTAACGG215550.025.5252884
CTCTTGA587550.025.3415134
TAACGGT221600.024.8606195
CCCGTAT62650.024.6329441
TTGAACT672450.024.4915587
GCCCTCT526850.024.2350641
CTTGAAC660250.024.0109026
CCTCTTG644750.022.9586753
TCTTGAA693800.022.5259235
CCGTATC96350.021.7614382
CCCTCTT611450.021.6186662
TATCGAA65650.021.420775
Produced by FastQC (version 0.11.5)