FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17020780
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1184920.6961608104916461No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG749400.4402853453249499No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC514490.30227169377666596No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA505990.29727779807975896No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG396500.23295054633218926No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA329810.19376902821139808No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC329480.19357514755492994No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA290360.17059147700634167No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA275930.16211360466441607No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC259480.1524489476980491No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT245650.14432358564061107No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA230760.1355754554139117No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA221330.13003516877604904No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC213990.12572279296248468No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA209930.1233374733707856No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA200430.11775606053306606No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG199810.11739179990576225No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTG192760.11324980406303355No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT191830.11270341312207785No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT190000.11162825675439081No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT178150.10466617863576169No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA173560.10196947495943194No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT171160.10055943382148173No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG240000.082.273963
TAGCATA171700.070.890879
CCAGTAG171000.070.8730555
CACCCGG282600.069.821044
GTAGCAT176800.068.845738
ACCCGGC299650.065.892175
CAGTAGC205950.059.296546
CTCACCC335300.058.9896162
GCCAGTA216300.056.134344
GAGTCTA164350.053.2478832
AGTCTAA164600.053.110943
AGTTACG294250.049.85465145
CCTCACC435600.046.110761
TGCCAGT267800.045.9073033
AGTAGCA267100.045.8669367
GTCTAAC195250.044.625574
GGAGTCT206750.043.719471
CGACCGT27400.040.8307151
TCAGACG220950.040.3294832
AACACGT222250.039.599128
Produced by FastQC (version 0.11.5)