FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17046366
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1177360.6906809345757331No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG750160.4400703352257015No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC515130.30219344111231683No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA509980.2991722693270812No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG399790.23453092582900073No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC339760.19931520888381724No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA318790.18701346668257623No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA299380.17562687554637746No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA274740.16117218180109474No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC264750.1553116951730357No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT251500.14753877747315763No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA236540.13876271341352167No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA226210.1327027707841073No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC212640.12474212978883592No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA211140.12386217684167992No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA204070.11971466528408459No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG203410.11932748598733596No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT196960.11554368831456512No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTG195100.11445254666009165No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT193970.11378964877323412No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA180630.1059639338965267No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT176400.10348246658554673No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT172250.10104793009841512No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG232400.079.937483
TAGCATA164500.069.0372859
CACCCGG275800.067.595024
CCAGTAG168850.067.565315
GTAGCAT168200.067.475558
ACCCGGC294500.063.2756965
CTCACCC325000.057.267122
CAGTAGC205100.055.9771656
GCCAGTA211950.053.964924
GAGTCTA162900.053.9441532
AGTCTAA165100.052.858813
AGTTACG280900.047.562263145
CCTCACC426600.044.3528481
GTCTAAC197150.044.30254
TGCCAGT259400.044.26123
AGTAGCA265700.043.2878537
GGAGTCT212450.042.234211
AACACGT221200.039.4149638
TAACACG224650.039.1645287
CGACCGT26100.039.0787771
Produced by FastQC (version 0.11.5)