FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19127518
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA859680.44944670814059623No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA472980.24727724736685647No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT364500.19056314572544122No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT337370.17637939224524582No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC303480.15866146355214514No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC274060.14328048207822885No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC265720.13892027183035457No Hit
CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT249410.1303932899187443No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG244360.12775311464874847No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC238750.1248201674676244No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA222040.11608406276235109No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG211320.11047957189218173No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG208480.10899480005717417No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC206910.10817399309204677No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTT105650.051.6771551
TGTTACG34400.042.1453863
GTTACGA36200.039.8491944
TTACGAC38200.037.7622575
CTTAACG222900.032.066043
TTAACGG224350.031.9231624
TAACGGT232450.030.9662045
TTCGCGC41750.030.731597
TACGACT47800.030.6331566
CCCGTCG130300.030.4480111
AACGGTT238000.030.152726
CCCGTAT42200.028.1178421
ACCCGGG615750.027.190287145
GAACTCT666250.026.523569
CTCTTGA627600.026.3808164
TGTCGAG35200.026.3594025
ACGGTTT276300.026.1042047
GCCCTCT537450.026.0464211
CCGTCGG150750.025.7942712
TTGAACT708000.025.606417
Produced by FastQC (version 0.11.5)