FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18902324
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT785610.4156155613457901No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA626470.3314248554833787No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA407310.21548144027157717No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC406490.215047631180166No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG400410.21183109547799522No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG356930.18882863292365532No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT344690.18235323868112727No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT303240.16042471814576875No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT296190.15669501803058714No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA295980.15658392058034767No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC282060.14921974673590402No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC272410.1441145543796625No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA271160.1434532600329991No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA258890.13696199472615112No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC257330.1361366993815152No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG255250.1350363055886673No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC249490.1319890612392423No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG247760.13107382986346017No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTACTA240190.12706903130006658No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAA229570.1214506745308143No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG217810.11522921731740499No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG214600.11353101343517338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG174950.070.562093
CACCCGG181950.067.807574
ACCCGGC197050.062.6482475
CGACCGT17800.055.5336721
AGTTACG154300.052.580616145
CCAGTAG113200.049.499845
TAGCATA121050.046.7086229
GTAGCAT129950.043.6791958
GTCTAAC107350.038.4896134
ACCGTTG27200.037.57693
CCCGGCC337350.037.0852246
GTAGGTC49350.037.0196532
CTCACCC345550.036.0018962
GAGTCTA120100.034.8298652
TCAGACG215000.034.6298752
CAGTAGC175950.032.37986
AGACGTG223800.032.292814
AGTCTAA131550.031.9049783
GTTGACT120150.031.7509381
CGGCCCG395550.031.375568
Produced by FastQC (version 0.11.5)