FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17292629
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA983560.5687741291390684No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA765550.44270307308391343No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC740450.42818821822870307No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG707410.409081811678259No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT592650.3427182760932418No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT568140.32854460706928945No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT557390.322328085567556No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA500980.2897072504128782No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT480050.2776038276192706No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG418290.24188918874047433No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAA398010.23016164864232036No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG394920.2283747601362407No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG343600.1986973756275No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA340070.1966560434506517No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG338880.19596788897743656No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC319290.18463936281753343No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG299880.17341492725021743No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA287180.16607075766212298No Hit
GACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCA240390.1390129864001593No Hit
GCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAAC236260.13662468558135377No Hit
AGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAA231120.13365232088192028No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC230080.13305090856919444No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT229990.13299886327290084No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA225930.13065104212899034No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA214720.12416851133508966No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG206450.11938612688677933No Hit
CTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTC205620.1189061535987385No Hit
CCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGA205230.11868062398146632No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG189650.10967100491197723No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA187920.10867057866100059No Hit
GGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAA179770.10395758794108172No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT178560.103257867846468No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCCGG131000.065.078264
TCACCCG135750.063.6526073
CCAGTAG107000.049.2550245
TAGCATA115400.046.5485349
TCAGACG283250.045.5173232
AGTTACG119850.045.0277145
GTAGCAT119500.044.525548
GAGTCTA112500.043.2742162
CAGTAGC127900.041.2618036
ACCCGGC209200.040.8902935
GTCTAAC126950.040.6924251
CGACCGT16500.038.0838971
CAGACGT341650.037.3855933
AGACGTG344750.036.9039424
GCCAGTA150500.035.1630254
GGAGTCT143550.034.714271
AGATCAG189850.033.5156942
TGCCAGT158950.033.1553543
AACACGT156000.032.9474265
ACGTGCG155200.032.835588
Produced by FastQC (version 0.11.5)