FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21369698
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT5724952.6790036995375415Illumina Single End PCR Primer 1 (100% over 50bp)
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA943170.4413586003882694No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG866890.40566319655055494No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC602340.2818664072838091No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTACTATTCGG573670.2684502139431264No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC529290.24768248947645402No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG516160.24153827536542632No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA484270.226615275517698No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG480600.2248978904615311No Hit
TGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCC423290.19807954235010716No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAA411940.19276828338893698No Hit
AGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAA379100.17740072882639707No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT372230.17418589630981215No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA371440.17381621396802147No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT364260.17045631622870852No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT360060.16849091643690986No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT359410.16818674742151246No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACT353240.16529948153689397No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA352420.16491576062516186No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA351520.1644946035269193No Hit
GACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCA345750.16179451857485305No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT322960.15112988494268847No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG321030.15022673694312386No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC298160.13952466712444883No Hit
ATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTC292080.1366795169496546No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA289510.13547687945800638No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG282390.13214505885857628No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA262450.12281408937084651No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTACTATTCGGCG259870.12160677235588449No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTACTA255490.11955714114443733No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC252000.11792398750791891No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA245620.11493845163371051No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG242160.11331933656713353No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC241810.1131555532511503No Hit
CATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACG238820.11175637578032221No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA238340.11153175866125951No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGC225730.10563087976254976No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA225570.10555600738952886No Hit
CTCTACAAACCACAAAGACATTGGAACACTATACCTACTATTCGGCGCAT213750.1000248108326098No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTC85500.059.1436962
CGACCGT44050.049.4121551
AGTAGGT107650.047.4949461
CACCCGG123200.044.072664
TAGGTCT123950.041.641033
CTACCGT133500.040.9422768
TCACCCG140350.039.8742833
GTCTAAC143200.038.7134781
GGTCTAC133650.038.1315545
AGTTACG85500.037.5797145
TCAGACG435800.036.308662
CAGACGT439750.035.689473
TCACATG216350.035.0094572
CACATGC271850.034.4446751
CAGATCA231700.034.3923721
AGACGTG456150.034.311774
ACCGTTG65350.033.944063
ACGTGCG163600.033.6753548
AGGTCTA158900.032.528484
ACATGCC287800.032.418432
Produced by FastQC (version 0.11.5)