FastQCFastQC Report
Wed 11 May 2022
EGAF00004828161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21525265
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT5567992.586723090284835Illumina Single End PCR Primer 1 (100% over 50bp)
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA919540.4271910241290874No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG848170.3940346379010897No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC592110.2750767528297561No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTACTATTCGG571640.2655669976653017No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC512380.23803655843493682No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG504170.2342224358213476No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG486000.225781192473124No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA462350.21479410358014173No Hit
TGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCC419460.19486868105921112No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAA404390.18786760581112474No Hit
AGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAA380250.17665287744424982No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT368950.17140323243407224No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT362300.16831383957410048No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA362070.16820698839247739No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT359840.16717099650108835No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT354570.16472271073085512No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA346970.16119197603374455No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACT345010.1602814181381739No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA341320.15856715352865575No Hit
GACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCA334790.15553350911126992No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG327530.15216072833481958No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT318920.14816077757927718No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC294770.13694140350885342No Hit
ATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTC286600.13314586370945955No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA283330.13162671864899222No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG275640.12805417261994217No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA262410.12190790682484048No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTACTA257760.11974765467463466No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTACTATTCGGCG255300.11860481160162256No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC255120.11852118893774363No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC247310.11489289446610762No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA243640.11318792126368711No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG240600.11177562738484288No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA238120.1106234929047331No Hit
CATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACG235880.10958285530979525No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA231830.10770134537251923No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGC231010.10732039768151518No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)