FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11234308
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC117080.1042164768849136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAA6550.030.8038545
CGACCGT11400.030.7516421
GTAGGTC43200.028.5632322
ACCGTTG14950.024.792333
GGTCGTA8150.024.7590124
GACCGTT15400.024.3727552
CGCAAAA27950.024.1352141
AGTAGGT50000.023.9024121
GCGATTT20150.022.83118494
TCGTAAG9050.022.8166436
TAGGTCT54300.022.7241343
TCAGACG71700.022.4445192
CAGACGT69650.021.8250543
GTTGACT56900.021.0972271
CGCGGTC10100.021.0362281
GGTCTAC62450.020.2114645
GTCTAAC28650.019.982864
GACTATT59250.019.958814
TTGACTA56900.019.8719332
CGAGCCG58100.019.55314494
Produced by FastQC (version 0.11.5)