FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7253977
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA195290.26921783733254184No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA130060.17929475100348402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAA9950.038.2096371
CGGTAAT11300.033.4791872
GTATCGA13200.031.4908544
CCCGTAT14900.029.9259761
TATCGAA15750.025.7992485
CTTCGCG17050.024.6539486
TTCGCGC17750.024.207947
TTAACGG58600.022.2369354
AGTTTAC111500.022.17070694
CTTAACG60200.022.1114233
AACGGTT58750.022.0211646
TAACGGT61250.021.2748455
CGTATCG23900.021.105433
CCGTATC25150.020.0564522
GTCGGTA4001.8189894E-1218.7746121
CCCGGTA9000.018.2530941
TCTTAAC73650.018.1368392
TCGACCG5000.017.7561830-31
ATCGAAG25800.017.5598326
CGCGGTT2957.2797775E-817.5017571
Produced by FastQC (version 0.11.5)