FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11143708
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC145100.1302080061681444No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG127000.11396565667370323No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT122010.10948779347053962No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA120710.10832121588254108No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA120230.107890479542357No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA113910.10221911772993333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT10300.037.3947941
GTAGGTC46100.029.3434032
GCGATTT18900.025.59834794
GACCGTT15300.025.4803622
AGTAGGT54450.025.1894951
ACCGTTG15900.024.5188393
TAGGTCT58750.023.7447473
CGAGCCG57900.022.14728794
CGCAAAA30050.021.5709441
TCAGACG70150.021.4260062
GTCTAAC30650.021.3017334
GGTCTAC66300.020.9705945
GTTGACT56250.020.2081891
AACACGT31750.019.5281378
CCGTTGA19850.019.4036734
AGGTCTA72500.019.3715254
GTCGTAA5600.019.2917845
CAGACGT74650.019.0647773
TTGACTA57250.018.7058872
CTCGATT16200.018.26760148-49
Produced by FastQC (version 0.11.5)