FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8626342
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA331430.38420688630244426No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA140490.162861616198384No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC109540.12698314071016428No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC97840.11342003365969028No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC94000.10896855237132957No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC92430.10714854569874462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAA14200.031.9167671
CGGTAAT15050.028.4145052
CGCGTAT1301.0901167E-525.3040433
GTCGGTA5150.024.7509861
GTATCGA18100.024.1456874
CCCGTAT19750.023.4258271
TCGGTAT5950.022.9042572
TATCGAA21100.021.1580855
AGTTTAC209950.020.70792494
CGCTAGT2109.14255E-720.232951
ACGCGTA1405.219375E-420.1399522
TGTCGAG10250.018.7972897
TTCGCGC16550.018.7404987
TCGACCG6050.018.26425630-31
CTTCGCG17600.017.8894636
CGTATCG32350.017.7223263
GAACTCT253300.017.661889
TTGAACT269000.016.945517
TCAATCG29450.016.5952288
CCGTATC35000.016.514762
Produced by FastQC (version 0.11.5)