FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8625600
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA135100.15662678538304584No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC130670.1514909107772213No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA100020.11595715080690039No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA99150.11494852531997773No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG93890.10885039881283622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT8700.043.374421
GACCGTT13300.028.5722272
ACCGTTG13800.027.8761563
GTAGGTC31700.027.2313562
GTCTAAC27050.026.8875224
GAGTCTA27950.026.0172022
CGCAAAA24050.024.9722671
AACACGT30300.024.1609348
TCAGACG69800.023.5918712
CCGTTGA17300.023.3259184
GTTGACT40900.023.2607841
AGTAGGT36950.022.8705861
CAGACGT69750.022.5992073
GCGATTT13900.022.28881694
TAGGTCT41800.021.54883
GGTCTAC44050.020.1314265
CGAGCCG51700.020.06594794
TAACACG37250.020.0315827
AGACGTG80100.019.9174064
GACTATT45900.019.5257194
Produced by FastQC (version 0.11.5)