FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5539149
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA114310.20636744019704112No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC109570.19781016903499074No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA82170.14834408679022718No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA81450.14704424813270053No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG80330.1450222768876591No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG76450.13801759078876558No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT66480.12001843604495925No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG65220.11774371839428766No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA57940.10460090530151835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT6350.036.566491
GTCTAAC18000.029.676894
AACACGT19550.027.5583528
GAGTCTA20250.027.2950292
GACCGTT8400.026.5413862
GTAGGTC20150.025.586422
CGCAAAA15450.025.5492081
ACCGTTG8950.024.9101243
TCAGACG46450.024.3986452
CAGACGT46100.023.6768883
TAACACG23550.022.8756647
GTTGACT25450.022.444211
ACACGTG25250.022.2567659
AGTAGGT23700.021.5541731
AGACGTG49800.021.35994
GCGATTT10200.020.50133594
CCGTTGA10950.020.3619884
GTCGTAA2301.1445627E-720.1947145
CGTAACG1150.00424543820.1910631
TAGGTCT25650.020.0998743
Produced by FastQC (version 0.11.5)