FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8519092
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA327430.3843484728184647No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA141960.16663747732739592No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC110130.12927434050483316No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC100570.11805248728385609No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC96080.11278197253885743No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC92300.10834487994729955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAA12350.032.4783061
CGGTAAT13450.028.970322
TTCGCGC16450.028.2514847
CGTACGT1057.2268864E-526.9651811
CTTCGCG17450.026.6324886
GTCGGTA5250.025.1675011
GTATCGA19900.024.7689864
CCCGTAT21250.024.6493471
TATCGAA22200.023.2601075
TCGGTAT6000.021.9080772
AGTTTAC204350.020.846394
CGTATCG31050.019.8053113
CCCGGTA13800.019.4911371
CGCTAGT1751.0460332E-418.8756261
CCGTATC32900.018.8354282
TCGACCG6200.018.55555230-31
GTTCGAC6350.018.48832728-29
CCCGTCG10800.018.3513031
GAACTCT247400.017.9309849
TTGAACT262550.017.361187
Produced by FastQC (version 0.11.5)