FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10278226
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG200910.19547147533047043No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA176680.17189736828125787No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC105480.10262471364221804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT59350.032.82871
GTATCGA60400.032.7552074
TATCGAA63800.031.4515725
CGTATCG77600.026.8301873
CCGTATC79700.026.0642742
ATCGAAG86250.024.7361416
CGCGGTT2803.6379788E-1223.588181
CCGGTAA9300.023.3345431
CGGTAAT8850.022.3042532
AGTTTAC106800.021.74100594
TCGAAGG99700.021.729067
CGCGTAT1150.00387575620.511461
TCAATCG31250.020.1506928
CTTCGCG16900.019.7427126
CGCGTTT2401.5216756E-719.6568161
TAGGTAT46750.019.60545394
TCGACCG8250.019.09472830-31
GTTCGAC8400.018.7537528-29
CGAAGGC109850.018.5234998
GTCCGAT4350.018.4367371
Produced by FastQC (version 0.11.5)