FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10278226
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC268760.2614848126515218No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG236110.22971863043291713No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG214460.20865468418382704No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC193020.18779505334870045No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT183460.17849383736064958No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA181500.17658689349699064No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT156920.15267226075783893No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA141730.13789344581448199No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA128900.12541074695185728No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT116910.11374530974508637No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACA113680.11060274409221982No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG106330.1034517046034987No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT105680.10281929975075464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT17150.057.578111
CGCAAAA43400.050.0313451
ACCGTTG24550.043.7648543
GACCGTT26150.040.728242
CCGTTGA30750.034.945664
TCACATG89250.034.4329762
GCGATTT21300.033.93978594
TAACCCC67050.033.872679
GTAGGTC59950.032.009262
ATCACAT92500.031.3945431
AGTAGGT62950.029.9392681
GTTGACT79500.029.0489671
TTAACCC80300.028.6929478
ACATGCC120400.027.7066862
TAGGTCT72650.026.8011843
CGAGCCG101650.026.46154694
GTCGTAA6350.025.8627015
GCAAAAT93700.025.0364022
ATCGACC52650.024.83236542-43
CACATGC129000.024.7339421
Produced by FastQC (version 0.11.5)