FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10257755
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC303010.2953960198893422No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG265440.2587700720089337No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG244260.23812227919266937No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC214790.20939279598703614No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT208060.2028319062016981No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA200850.195803077768966No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT175060.1706611241933542No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA158890.15489744100926567No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA131730.12841991254421656No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT130580.12729880953483486No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACA127690.12448142892864959No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG121320.11827149312885715No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT120110.11709189778855118No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT112840.11000457702489481No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT111730.10892246890279599No Hit
AGGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACAC111020.10823030965352555No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC106780.10409685160154439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT22000.055.708511
CGCAAAA46550.052.053871
ACCGTTG26750.044.900733
GACCGTT28600.042.365782
CCGTTGA31800.036.9075364
TAACCCC64850.036.0440869
GCGATTT20650.033.48212494
TCACATG85050.032.948372
GTAGGTC56850.030.859032
ATCACAT99100.028.7938121
TTAACCC82450.028.5752968
AGTAGGT62700.028.126571
ACATGCC120250.028.0110172
CCGTATG46650.027.2471164
GTTGACT80650.026.7966541
ACCGTAT48200.026.554583
GCAAAAT94250.026.208012
CGAGCCG100800.025.77948894
TAGGTCT68200.025.7030583
ATCGACC52650.025.6885342-43
Produced by FastQC (version 0.11.5)