FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10188137
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG196950.19331306597074618No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA172440.1692556745163517No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC102820.10092129699473024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT60850.034.9847371
GTATCGA63500.033.5640684
TATCGAA65350.033.1167565
CGCGTAT1401.8166247E-830.34431
CGTATCG79050.028.3275453
CCGTATC80850.027.7565772
ATCGAAG86350.026.422046
TCGAAGG99700.023.0253457
CCGGTAA9250.022.452961
CGATCGT1703.3298584E-622.212821
AGTTTAC106850.020.13086794
CGCTAGG4300.019.7590791
CGAAGGC109800.019.7102018
CGGTAAT9550.019.6641252
TCGCGAT3153.7471182E-1019.38225794
TCGACCG8000.018.19676630-31
ACGCGTA1300.008094531518.0569612
GTCCCGT8450.017.8754041
TTCGCGC15300.017.796277
GAAGGCC125850.017.5322279
Produced by FastQC (version 0.11.5)