FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12096055
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA823780.6810319562865744No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA407020.3364898721111966No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC283520.2343904686279948No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC277980.22981046299806013No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC250880.20740646433899315No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC250380.20699310643015428No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT211390.17475945669889892No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT206080.17036959570703009No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA173130.14312930951454833No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC170420.14088890964864165No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG160650.13281189610993005No Hit
AACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT149020.12319719115033785No Hit
GCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCC137710.11384703525240253No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG133480.11035002734362566No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC127920.10575348739733739No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG122630.10138016072182211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTA9200.035.398711
CCGGTAA16150.034.193181
TTCGCGC31850.033.0501827
CTTCGCG32250.032.203116
TCGGTAT9350.031.6638872
CGGTAAT19100.028.540392
CGCTAGT1503.5555786E-828.3189661
CCCGTAT31850.026.6740671
GTATCGA30850.026.6574234
TATCGAA32750.025.5413595
CCCGGTA16750.024.7967071
CGCGATT1252.3571527E-422.6551721
TCGACCG15950.022.40574330-31
CCCGTCG18550.021.8816811
AGTTTAC528100.021.55504494
CGTACGT1100.002987360821.4537621
ACGACTT19450.021.1118241
CGCTAGG6050.021.0636941
CCGCCCT39100.021.0038381
CGTAACG1354.0841175E-420.885883
Produced by FastQC (version 0.11.5)