FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12467586
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA838900.6728648192200158No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA431430.34604132668505355No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC276590.22184727660992273No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC253870.2036240215226909No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC249610.20020716119383497No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC244520.19612457455677468No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT223420.1792006888903754No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG222300.17830235941424427No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA181990.14597051907241707No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC169930.1362974356062192No Hit
AACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT139510.11189816537058576No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG137810.110534629558601No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT135420.10861765862292828No Hit
GCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCC130630.10477569595268885No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC128610.10315549457609516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTA8200.041.4507641
CCGGTAA11200.037.0918541
TCGGTAT8850.035.5767142
CGCGTAT1151.0955773E-732.840221
CCCGTAT70550.032.787851
TATCGAA74300.031.4974085
CGGTAAT13450.031.4452132
GTATCGA73800.031.3924224
CTTCGCG30900.031.0245866
TTCGCGC32350.029.9245247
GTCGTAT2301.4551915E-1024.6301651
CGTATCG105400.024.4774423
ATCGAAG101200.024.4717146
CCGTATC107950.023.8992352
CGCGTAG3350.022.5470161
AGTTTAC510400.021.66712694
CCGCCCT28100.020.9999181
TCGAAGG122700.020.4517757
CGCTAGG4400.020.3851931
CGGGCTA8200.019.573971
Produced by FastQC (version 0.11.5)