FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004828222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12672392
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1527071.2050369022675433No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG1036860.8182038560675838No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG520770.4109484618215724No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT508620.40136069023117343No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG506560.3997351092043238No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC426530.33658207542822227No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG346890.2737367972834174No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT340110.26838658400087373No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA325640.2569680609627606No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA324340.25594220885843805No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA299920.23667197163724105No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG296300.23381536808520445No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG290840.2295067892470498No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC270580.21351927875968482No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG257480.20318184601612702No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC253730.20022265725365818No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT251690.19861285856687516No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT241690.1907216885336249No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG193480.15267835780332553No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC187130.14766746483221163No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG182570.14406909129704953No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG180950.142790721751663No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC174940.13804812856167958No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT160370.126550693823234No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAG151700.11970904940440605No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT150930.1191014293118458No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT144250.11383012772963462No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA143600.11331720167747335No Hit
AGGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACAC142070.11210985266238607No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACA140730.11105243587793054No Hit
CGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACC140140.11058685684596878No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC136360.1076039945734002No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT129090.10186711395922728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)