FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12698464
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1322531.0414881673878038No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG883150.6954778152696263No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG453460.3570983073228384No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT432380.3404978743885875No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG427250.33645801570961653No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC364850.2873182142344145No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG297900.23459530223497896No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT291810.2297994466102357No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA286180.2253658395220083No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA273230.2151677557222669No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA261640.2060406675957029No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG254970.2007880638162222No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG250290.197102578705582No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC228450.17990364818926133No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG225600.17765928225649968No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC219780.1730760507727549No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT215540.16973706426226037No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT210920.166098828960731No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG165890.1306378472230972No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC159540.12563724242554059No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG156670.1233771265564087No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG155460.12242425540600817No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC148480.11692752761278845No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT134920.10624907075375414No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAG130210.10253996073855863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT27050.061.374251
CGCAAAA55450.058.63651
ACCGTTG36400.047.686883
GACCGTT37450.046.099682
GCGATTT35750.042.9385994
CGGTGTG296400.040.1384161
TAACCCC91500.036.923039
CCGTTGA48450.036.4158334
CACCCGG48400.033.6418844
TCACATG93900.033.6241572
ATCACAT93350.032.1596451
TTAACCC105400.032.0549358
GTTGACT103700.031.7118851
GGTGTGA397350.031.5947442
AAATTAA107950.031.3820745
GCAAAAT111700.030.9119642
GTAGGTC50050.029.7137282
GTCTAAC33800.029.1540414
CAAAATT118300.029.0285193
TCAGACG139000.028.5196292
Produced by FastQC (version 0.11.5)