FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12666468
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA836590.6604761485206452No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA562650.444204335415366No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC383290.30260211449632207No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC277270.218900801707311No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC270800.21379282685591597No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC258630.20418478142446655No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG227710.17977387224283833No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT212330.1676315765373583No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA204160.16118147537261374No Hit
GTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCC194840.15382346523119153No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT191080.150854997620489No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC178660.14104958067237056No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC169210.13358893734228042No Hit
CTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAA165820.13091257957624808No Hit
AACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT157610.12443089896883647No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA157100.1240282610748316No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG156680.12369667692682759No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG141890.11202017800068655No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG137440.10850696500397743No Hit
GCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCC137400.10847538556131037No Hit
GGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACG130320.10288582420924287No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA128210.10122000860855608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTA10450.037.8399471
TCGGTAT10650.034.0669942
GAACGGG69200.032.225089
AGGAACG69900.031.7124737
GTCGGGA71150.031.0979121
GTATCGA58450.030.506344
TATCGAA61550.029.1855285
CCCGTAT62500.028.506321
CGCGTAT1454.600588E-827.5772721
CCGGTAA11600.026.4282191
CGTATCG87250.024.9499233
CCGTATC87100.024.9420952
CGGTAAT11950.024.8071252
CCGCCCT30850.024.4833351
GGAACGG93300.023.853718
TGTCGAG21400.022.9497957
GGGTCGG69050.022.747532
TAGGTAT52700.022.15889294
ATCGAAG90900.022.0984366
ACGTTCG6550.021.6161466
Produced by FastQC (version 0.11.5)