FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004828226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12666468
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1335441.0543112728820694No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG956260.7549539461197865No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG473380.3737269142431813No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT458420.3619162026857053No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG454620.3589161556323357No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC415060.3276840868346251No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG334390.26399624583585574No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT327730.2587382686317922No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA315950.24943812276634653No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA311270.24574332797430193No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG292610.23101151797012398No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA289300.22839831908942573No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG279490.22065346077533216No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC259420.20480847541714076No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC247080.1950662173543564No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT244330.19289513067099684No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT234550.18517395693890357No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG232220.183334454403548No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG186750.14743652295178103No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG174970.13813637708633533No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC173100.13666003814165084No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC170710.13477316644229473No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG163850.12935729202489596No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT153720.12135979816946603No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAG147310.11629919248207156No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT146050.11530444003805954No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA141800.11194912425468567No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT141380.11161754010668168No Hit
CGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACC136530.10778853268330209No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACA135760.10718062841196141No Hit
AGGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACAC135750.10717273355129464No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC130930.10336741070991534No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGGCTTCCCGGA129780.10245950173323772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)