FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004828228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8157789
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1140021.3974619838782298No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG831591.019381599597636No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG383530.47013964200348896No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT380570.46651120787752665No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG370500.4541671769152157No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC304570.37334871004876446No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT241790.29639158355284745No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG233210.2858740278769162No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA226210.27729327149795124No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA222020.2721570758939708No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG213630.2618724264626114No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA211730.2595433640168923No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG197150.24167087430184822No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG192800.23633854712349142No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC186180.22822360323367033No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT173390.2125453355069615No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC165280.2026039163307607No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT163280.20015227165105642No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG138450.16971510295252795No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC137040.16798669345333644No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG136240.16700603558145471No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC121880.1494032267811781No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG120270.14742965281401615No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT107330.13156751173632955No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT106950.13110169924718573No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAG106160.13013329959870254No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT103950.12742423222762933No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA103330.12666422237692102No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC100460.12314611226154537No Hit
CGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACC98980.12133189519856422No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACA93850.11504342659512277No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG93380.11446729009539226No Hit
AGGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACAC92650.11357243978730022No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT88400.10836269484292864No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT86890.10651170310975193No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC86700.10627879686518002No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG84960.1041458659938373No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT82790.10148583151635816No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT82690.10136324928237295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)