FastQCFastQC Report
Wed 11 May 2022
EGAF00004828230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12862538
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1527001.1871685043807063No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG1063260.8266331263705499No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG523880.4072913137360605No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG516350.40143710362604956No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT514080.3996722886260861No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC458350.3563449141996704No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG364580.28344328312188466No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT353650.2749457377696377No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA343250.2668602417345628No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA338500.2631673469108507No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG320040.2488155914485928No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA313960.24408868607424133No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG302790.23540455235195418No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC278380.21642696021578323No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT270440.2102539949736203No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC263920.20518501092086178No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG258160.20070689003989728No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT252990.19668746556861486No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG208650.1622152642036898No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC196360.15266038475454843No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG189340.14720267493087288No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG185730.1443960748648517No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC183180.14241357343317468No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT169360.13166919312502712No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAG157490.12244084332345606No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT153740.1195254000415781No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA152270.11838254627508195No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT150530.11702978059229058No Hit
CGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACC147940.1150161810989402No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACA147550.11471297499762488No Hit
AGGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACAC146940.11423872955710607No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC143880.11185972783909365No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT137630.10700065570263038No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC129760.10088211206839583No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)