FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12372178
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA148990.12042342100154071No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG140030.11318136547986944No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA127430.10299722490251918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA19000.028.6907884
CCCGTAT24050.025.5163761
CCCGTCG23200.022.5852931
ACGACTT22050.022.4787121
TATCGAA25250.022.3335635
TCGACCG18350.021.78141830-31
GTTCGAC19600.020.39229428-29
CGACCGT20050.018.75660132-33
CGTATCG43050.018.011463
CGCGTAT2102.066014E-517.982971
CGATCCG22050.017.79588182-83
GTCCGTT8950.017.4053041
AAGTTCG23900.016.72338926-27
CCGTATC47200.016.5273882
CGCGTTT2901.1004795E-616.2776871
TGTCGAG13300.015.9000697
AGTTTAC134600.015.71265294
CGACTTT39800.014.9954252
ACGTACG8050.014.88645254-55
CCGGTAA11750.014.8646461
Produced by FastQC (version 0.11.5)