FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12406306
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT325460.2623343322339462No Hit
CCCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTAC314780.25372580685983404No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT210870.16997001363661352No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT188840.15221291494825293No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC187690.15128596699130265No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT132700.10696173381504535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT49700.044.29810394
TCACCCG59050.040.6835483
ACCCGGC57350.040.5011025
CGACCGT11300.038.485681
CACCCGG67700.035.0743984
CCCTAGA119300.034.0082861
CGGCCCG80550.029.7126778
CCTAGAG143150.029.4345422
TAGAGGC153350.027.6906783
CTCGATT49650.026.84982748-49
ATTCCGT49950.026.40285952-53
GTAGCAT49450.025.4334748
CTAGAGG170050.025.2474062
TAGCATA49850.025.2283739
CGGACAC53900.024.91137712-13
CCAGTAG50600.024.6675595
ACCGTTG18200.023.97623
TCTTGGC95900.023.6370746
CGGACAG57350.023.5278818-19
CGTTCTT57800.023.02888376-77
Produced by FastQC (version 0.11.5)