FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12525444
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA151780.12117734109864688No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG145280.11598790430103714No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA132060.1054333882295909No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC127470.1017688474755865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA20750.030.768834
CCCGTAT24500.029.465521
ACGACTT22350.024.48891
TATCGAA26850.024.3030415
CCCGTCG22500.023.277121
TCGACCG20100.022.54452330-31
GTTCGAC20650.021.60434528-29
CGTATCG43350.021.4420473
CGCGTTT3101.2732926E-1121.308611
CGACCGT22150.020.24471532-33
CGCGTAT2109.1933E-720.2214371
CCGTATC46250.020.2002032
CGATCCG22700.018.8466382-83
AAGTTCG23800.018.84307726-27
CGCGTAG4950.017.1575831
CCCGGTT17700.017.0606481
CGACTTT41500.016.9690512
TTCGACC27050.016.57852630-31
CGGTAAT8700.015.6492382
AGTTTAC136100.015.01078394
Produced by FastQC (version 0.11.5)