FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12102870
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG168140.1389257258815471No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC161330.1332989613207446No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT157700.13029967272225512No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC153120.12651544633628223No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG127920.10569393871040504No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA127690.1055039011408038No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC122960.10159573720943875No Hit
CGATAAGGAGACCTGCTTTGCCGAGGAGGGTAAAAAACTTGTTGCTGCAA121750.1005959743432756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT12650.050.8117141
ACCGTTG16100.039.9359663
CGCAAAA45600.037.416041
GCGATTT32350.034.12789594
ACCCGGC40500.031.2822785
CACCCGG41200.030.8665524
GACCGTT20950.030.6862982
TTAACCC61650.027.3798798
TAACCCC64350.026.230759
TCACCCG53000.025.5118143
CTCGATT29000.024.78006248-49
GTAGGTC28300.023.8857692
CGGACAC32550.022.79197512-13
CCGTTGA28800.022.6526834
CGGCCCG56800.022.3091458
ATTCCGT33050.021.88001452-53
ACCGTAT36600.021.3134633
GTTGACT61950.021.1326481
TTCTCGA34400.020.95869646-47
AGTAGGT32600.020.8766271
Produced by FastQC (version 0.11.5)