FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8024365
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA140290.1748300332798919No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC133260.16606921544570818No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA125150.15596249672092433No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT124800.15552632513600764No Hit
GTGATGTTATAAGCCTAAGGCAGCTTGACTTGCAGCAACAAGTTTTTTAC104640.1304028418448064No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA102640.12791043278813963No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC99280.12372318557293942No Hit
CTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCA84240.10498026946680517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT1450.042.0918121
CCGGTAA9700.030.9763661
CGGTAAT9600.030.1646982
GTATCGA22800.026.0164094
CCCGTAT25250.024.3574681
TATCGAA24100.024.225435
CGCTAGT1201.8530813E-423.474281
GTCGGTA3450.023.1340711
CGGGCTA6850.022.6175541
CGTATCG32500.022.4191823
CGCGTTT1805.7256966E-620.8660241
CCGTATC35000.020.8178122
ACGCGTA1150.0041069120.307232
CGGGTAA3553.6379788E-1219.8374181
CCCGTCG10000.019.7183931
CGCTAGG4050.019.70681
TCGACCG5000.019.15938830-31
TCAATCG26250.019.0385118
CCCGGTA11900.018.9372331
GTCGTTT6100.018.4715611
Produced by FastQC (version 0.11.5)