FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10800316
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG173670.16080085064177752No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA119300.11045973099305614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA17750.031.505064
CCCGTAT21850.028.7335011
CGTATCG25050.026.8262543
TATCGAA23750.024.535215
CCGTATC29000.023.9825532
CGCGTTT2502.3235953E-718.8820171
CCCGGTT18350.017.4928761
ATCGAAG36750.017.3905496
GTCCGTT8750.016.7240721
CTCCGTT19700.016.5337451
TAAGCCG67050.016.29505766-67
GAAATAA114650.015.66013194
GACGAAA71400.015.56649446-47
CTCCGGT22700.015.3884281
CTCCGAT41000.015.19772151
TCGACGA74550.014.9402444-45
GTCGATT9200.014.879851
GTCCTTA70750.014.41171551
CTCGCGA2308.379033E-414.3667521
AACGGTG54100.014.3323827
Produced by FastQC (version 0.11.5)