FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10800316
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTAC340430.31520374033500503No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT327320.30306520661062136No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT245240.22706743024926307No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC171800.15906941982067932No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT161740.1497548775424719No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT115820.10723760304791083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGA107800.037.622751
TAGAGGC130900.030.2342824
CCTAGAG133450.029.5469442
CGACCGT9700.029.238931
CTAGAGG139350.028.436443
GCATTCG20550.026.2319853
CATTCGG21350.025.469114
GCGATTT17800.025.33730594
CACACGC102300.024.2197384
ACACGCC103400.024.0990435
CCGGGTA158900.022.93718742-43
CCCACAC109400.022.6021392
ACCGTTG12900.021.993773
CCCCACA119500.021.2416551
CGACCTA6250.021.1767831
GTAGGTC19000.021.150982
ACGCCTT117550.020.960717
ATTCTTG81450.020.550674
GGCAGCT195350.020.4806528
AGAGGCA195800.020.4541035
Produced by FastQC (version 0.11.5)