FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10956660
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG175730.16038646813901317No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA123850.11303627200259933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA16700.027.2678664
CCCGTAT21650.025.2825761
CGTATCG23950.024.1099013
CGCGTTT3007.2759576E-1222.0205441
TATCGAA22350.021.2148385
CCGTATC28550.021.213252
TCGACCG5300.017.2777630-31
TAAGCCG70650.016.67973566-67
CGCTAGT2002.905969E-416.5154081
GAAATAA122100.016.31000994
CTCCGGT20300.016.0388891
ACGTACG3100.015.92498252-53
GTCGAAT18800.015.8126251
GACGAAA74950.015.7672846-47
TCGACGA77400.015.28879944-45
TCAATCG12650.015.2156048
GTCCGTT8100.015.1464061
GTTCGAC5900.015.12380228-29
ATCGAAG34600.015.0606166
CCCAAAT188750.014.8498051
Produced by FastQC (version 0.11.5)