FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11189301
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG157660.1409024567307645No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG128380.1147346022776579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA44000.036.6337134
CCCGTAT48000.034.893111
TATCGAA48500.033.7191775
CGTATCG52950.032.6627243
CCGTATC57800.030.6537782
ATCGAAG65050.026.0795066
CGCGTAT2007.7852746E-1025.948681
TCGAAGG78800.021.946297
GTCCGTT10150.020.9169831
CCCGTCG19000.020.8582691
TCGACCG10550.020.27937930-31
CTCCGAT80200.020.0012471
TCCGATT75950.019.615622
CGAAGGC90450.018.3402658
GTTCGAC12500.017.3038828-29
CGCTAGT2555.881786E-616.6515581
CCGATTA85050.016.4669233
CTCCGTT24650.016.4601611
CGATCCG13800.015.66498182-83
CGTACGC11250.015.0379785
Produced by FastQC (version 0.11.5)