FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11373779
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC233600.20538468348998165No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG227720.20021489779254548No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG158880.13968971966133684No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA148370.130449167334797No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACA146420.12873469758819825No Hit
AGGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACAC127330.111950478376624No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC121370.10671035545881452No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT120940.10633229289930815No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC118210.10393203525406991No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT115640.10167245204957824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT18850.049.1532971
ACCGTTG23550.038.9930083
GCGGGTC65450.036.3247872
GCGATTT35100.036.21989494
GACCGTT26750.034.6412
CGCGGGT76800.031.6231351
GTCGTAA12300.030.9248265
ACCCGGC45400.030.6523595
GGTCGTA12600.030.5590954
TCACCCG46000.030.2465533
CGCAAAA47700.029.9212881
TAACCCC47200.029.5734869
CACCCGG47550.029.0735934
CCGTTGA32100.028.6149564
TCGTAAG14150.027.207446
CTTAGCG17050.027.1995228
GTAGGTC38350.026.603582
CGCGGTC15650.025.415781
CTCGATT31100.025.05761748-49
GTCTGTC98850.024.68176
Produced by FastQC (version 0.11.5)