FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7477236
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG180810.24181395371230757No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC159490.21330074375076566No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG111460.14906577778205743No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA103050.13781830612274373No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACA100710.13468880746842815No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT96460.1290048889723422No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC91670.12259877847910645No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT89060.119108183826216No Hit
AGGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACAC87480.11699510353825932No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC86730.11599205909777355No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA84000.11234097733440539No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT79550.10638958032085652No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG75180.1005451747142928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT12000.039.8648721
GCGGGTC48800.035.882692
GCGATTT24800.034.47591494
CGCGGGT54550.032.9496351
TCACCCG29350.032.5999373
ACCGTTG15250.031.9799023
ACCCGGC28800.030.6406825
TAACCCC28150.030.5201769
CACCCGG30250.029.6349454
CTTAGCG10550.027.2920288
CGCAAAA33750.026.8346081
GACCGTT18250.026.4687822
CTCGATT20250.025.80881548-49
GTCTGTC69300.025.4667266
GCGAACG4050.025.2304693
CCGTTGA19650.025.0562174
GTCGTAA8500.024.5884345
GGTCTGT73300.024.2678955
CGAGCCG47400.023.82196494
ATTCCGT22450.023.79619452-53
Produced by FastQC (version 0.11.5)