FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11105751
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA142220.12805977731717558No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA122530.11033022440355453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA21300.034.6032374
CCCGTAT26900.031.7521741
TATCGAA31800.023.4728975
CGTATCG39350.023.1448143
CCGTATC41900.022.4098032
TCGACCG12300.020.6160130-31
AGTTTAC89050.019.67107494
TCTACGG25100.019.4516437
GTTCGAC13400.018.92497328-29
CGTACGT1751.0459629E-418.8758771
CGCGTAT2001.3611521E-518.8758771
CGATCCG12700.018.51059782-83
ATCGCGT4850.018.3977694
CGACCGT13800.017.69294532-33
CTCCGAT59800.017.124021
TACGGAG30250.017.0711989
ATCGAAG54300.016.3402486
GTCCGTT7850.016.230851
TCAATCG17650.016.2248928
AAGTTCG16200.015.79921826-27
Produced by FastQC (version 0.11.5)