FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13522169
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG333220.24642496333243577No Hit
CTTCAAATGGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAG251820.186227520155975No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT220530.16308774132315607No Hit
GGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAGGCTTTGAA195220.14437032993745308No Hit
CTTACTTTCAACAAAATCAGCAGCTAATGAAGGCAAGTCAGCAGGCATCT189940.14046563092060155No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA178180.13176880129215957No Hit
CTCCAAAAAGGGTATGAAGTGATTTGTCACAATTTTCAGCTGACTCATCA172400.12749433911083347No Hit
CTCAGGTTCTTGTTTTGCACAGCAGTCAGCCATTTCACCATAGGTTTCAC163110.12062413951489587No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA162490.1201656331909474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA28500.035.621884
CCCGTAT31500.034.0587541
TATCGAA40500.026.343825
CGTATCG48450.025.6105023
CCGTATC52000.023.9524782
CTTACTT101150.020.0965861
ATCGAAG59850.018.7685386
GAAATAA169500.018.57791594
ATCGCGT6550.017.93868494
AATGGAC143250.017.5203726
TAAGCCG115950.017.4094266-67
CCCGGTT15900.016.8687231
GACGAAA121150.016.5658646-47
CGACGCA6600.016.3791684
TCGACGA122700.016.35659444-45
AACGGTT45750.016.3343836
CCCGTCG17400.016.2258131
CGAAACA130200.015.90173448-49
CTTAACG49600.015.7302053
CGAAGGC65850.015.559168
Produced by FastQC (version 0.11.5)