FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13522169
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT602080.44525401213370425No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT519430.3841321610460571No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT331600.24522693067953816No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT326920.24176594746005614No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT262000.1937558981846773No Hit
CCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTT257590.19049458707401157No Hit
GTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCC218590.16165306024499473No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC196920.14562752469666665No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC192520.14237360884929037No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN185430.13713036717704088No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT177970.13161350076308023No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA171170.126584721726226No Hit
CAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTT168910.12491339222280094No Hit
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG153200.11329543359500979No Hit
GACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGG149890.1108476014461881No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG146340.10822228297841863No Hit
CTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCTTTA144200.10663969663446744No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA143920.10643262926236167No Hit
GGCAAGACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGC136690.10108585390405933No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT135430.1001540507295834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT15850.048.6364971
ACCGTTG21600.036.559353
GTCTAAC40900.036.20221
GACCGTT21950.035.7626462
GCGATTT32150.030.11564494
CCGTTGA26350.029.4335754
ACGTGCG49300.029.170368
AACACGT51800.027.947295
ACTACCG46300.027.5076837
ACACGTG54350.026.4596636
TAACACG55550.025.97734
CGTGCGC57050.025.7019629
ACCCGGC39750.025.65915
GTAGGTC29950.025.1113972
TCAGACG43100.025.0840872
CCGTATG53000.024.6552054
CTACCGT56000.024.5938951
GTTGACT87850.023.970881
CGTATGG55000.023.757515
CCCCACA248200.023.5405751
Produced by FastQC (version 0.11.5)