FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12478224
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA130190.10433375775270584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA29000.033.387174
CCCGTAT31600.033.2100641
CGTATCG46800.026.8148753
TATCGAA36900.026.6213635
CCGTATC50000.025.7567422
CTTCGCG16950.021.351056
ATCGAAG57800.019.0276976
CGGTTCG6050.018.6451643
ACGACCG16300.018.4537894
GTCGCGA1902.0052357E-417.3378961
CCGTTAA19200.017.2574130-31
ATCGCGT4951.8189894E-1216.14123294
GTCCGTT11400.016.0994741
GTCCGCT16400.016.069271
TCGAAGG69250.015.9490247
TTGCACG36750.015.4744088
ACGGTTA22200.015.348680512-13
GGTACCG21950.014.98865220-21
CGAAGGC66800.014.9157618
AGTTTAC106900.014.64062194
Produced by FastQC (version 0.11.5)