FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14051727
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA190190.135349911082104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT28150.031.103961
GTATCGA24150.029.5820734
TATCGAA33650.022.068575
CGTATCG49000.021.5818463
CCGTATC54650.019.7806192
CGGTTCG8550.017.0411053
ATCGCGT5650.016.63679994
AGTTTAC147600.016.36684694
ATCGAAG64900.014.918596
GTCCGAT14700.014.4104091
CTCGTAT13850.013.9352641
CCCGTCG17950.013.8992871
GTCGGTT31550.013.8760131
GAACTCT184300.013.7710159
AACGGTT63700.013.5763696
TCGACGA52900.013.50462144-45
ACCCGTT37400.013.4466572
TCGAAGG73000.013.391777
TCGGTCG17250.013.3507157
TTAACGG64350.013.2930614
Produced by FastQC (version 0.11.5)