FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14023499
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN211040.15049025924271825No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT204460.14579813497330446No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT203330.14499234463524405No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT155970.11122045931618064No Hit
CAAGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGA143400.10225693316625187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT16100.030.6566681
ACGTGCG30050.028.7746948
GCGATTT37900.027.40692394
GTCTAAC33950.027.1379991
TAACACG36300.024.6018714
TCACCCG48750.023.718453
ACCCGGC49450.023.0015875
CATTCGG26450.022.568324
AACACGT39500.022.251195
CGACCTA12950.022.1401844
GACCGTT22250.021.971762
GCATTCG28900.021.4685123
ACCGTTG23700.021.0224513
CTCGATT30650.020.55631448-49
CACCCGG57750.020.1032434
GTTGACT76000.019.7305241
TCAGACG39200.019.666152
CCGTTGA25100.019.662414
TTGACTA72050.019.1811582
ACTACCG32550.018.6237357
Produced by FastQC (version 0.11.5)