FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14019246
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA189530.13519272006497352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT26750.030.0840261
GTATCGA23650.029.213954
TATCGAA33450.021.7790875
CGTATCG46950.020.9225583
CCGTATC52300.019.5012342
AGTTTAC144250.016.68185294
CCCGTCG18200.015.2561261
TAAGCCG51650.014.78676666-67
TATCGCG3200.014.68734586-87
CGCTAGT3859.060386E-814.66844751
TCGACGA53050.014.61875544-45
ATCGCGT7150.014.46128194
GAACTCT174900.014.376439
TCTACGG26050.014.2530047
GACGAAA54350.014.0961346-47
ACCCGTT35750.013.93586352
TTAACGG59450.013.91441254
AACGGTT57500.013.8140136
CGTGCCG110100.013.68133768-69
GTCCGTT15350.013.4898761
Produced by FastQC (version 0.11.5)