FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15033872
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA153430.10205621013668335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT44050.028.2151181
GTATCGA44800.026.333224
TATCGAA52000.023.5005635
CGGTTCG8000.022.9130483
CGTATCG82450.022.4032043
CCGTATC84350.021.619962
CCCGTCG30800.020.0237031
TTGCACG46500.018.4967258
CCGTTAA27650.018.01767730-31
ACGGTTA28550.017.69682912-13
ACGTTCG11350.017.3925446
GTCGCAA6750.016.7390691
ATCGAAG86350.016.6014966
AGTTTAC105850.016.29533494
TCGACCG12300.015.66631230-31
CGGCATG50150.015.3702573
ACGACTT21250.015.286711
GTCCGCT23650.015.1288621
ATCGCGT7150.015.11853994
TACGACG14600.014.8081799
Produced by FastQC (version 0.11.5)